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Biolayer Interferometry (BLI) is a label-free optical technique for measuring biomolecular interactions in real-time. It analyzes the interference pattern of white light reflected from an immobilized protein layer on the biosensor tip and an internal reference layer. Only molecules binding to or dissociating from the biosensor can shift the interference pattern, making BLI unique and ideal for protein binding, quantitation, affinity, and kinetics in crude samples. Bio-Layer Interferometry measures biomolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. In BLI experiment, one molecule is immobilized to a biosensor and binding to a second molecule is measured. The molecules that bind or dissociate themselves from the biosensor causes a shift in the interference pattern that is measured in real-time. The unbound molecules, variations in the refractive index of the surrounding medium or flow rate, however, do not affect the interference. This unique feature of BLI technology therefore allows measuring crude samples to determine the kinetics and affinity of molecular interactions. Anotheradvantage of BLI is that the number of sensors can be scaled up easily without making the system more error-prone or complex. Theoretically, there is no need to regenerate single sensors, because immobilizing an equal amount of ligand on additional sensors can easily create duplicates of the surface.

USD4480.00

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Biolayer Interferometry (BLI) is a label-free optical technique for measuring biomolecular interactions in real-time. It analyzes the interference pattern of white light reflected from an immobilized protein layer on the biosensor tip and an internal reference layer. Only molecules binding to or dissociating from the biosensor can shift the interference pattern, making BLI unique and ideal for protein binding, quantitation, affinity, and kinetics in crude samples. Bio-Layer Interferometry measures biomolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. In BLI experiment, one molecule is immobilized to a biosensor and binding to a second molecule is measured. The molecules that bind or dissociate themselves from the biosensor causes a shift in the interference pattern that is measured in real-time. The unbound molecules, variations in the refractive index of the surrounding medium or flow rate, however, do not affect the interference. This unique feature of BLI technology therefore allows measuring crude samples to determine the kinetics and affinity of molecular interactions. Anotheradvantage of BLI is that the number of sensors can be scaled up easily without making the system more error-prone or complex. Theoretically, there is no need to regenerate single sensors, because immobilizing an equal amount of ligand on additional sensors can easily create duplicates of the surface.

USD3480.00

Add To Cart

Bio-Layer Interferometry (BLI) is a label-free, real-time monitoring optical detection technology, mainly used to analyze the interaction between biomolecules. BLI instruments detect the kinetic changes or concentration data of molecular interactions by monitoring the changes in the film thickness caused by the binding of biomolecules on the surface of the biosensor.

USD3480.00

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Bio-Layer Interferometry (BLI) is a label-free, real-time monitoring optical detection technology, mainly used to analyze the interaction between biomolecules. BLI instruments detect the kinetic changes or concentration data of molecular interactions by monitoring the changes in the film thickness caused by the binding of biomolecules on the surface of the biosensor.

USD4480.00

Add To Cart